ssr_primer
Primer Details for TRINITY_DN9989_c0_g1_i1 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAGCAATAGTTGAGGCGA | TCCCACGACACAAGACTTCG | Start: 3267 |
| Tm | 60.036 | 59.968 | End: 3391 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
|---|
| Sequence | AGGCTTGAAGGGCTTGATCC | ACAGCCGTTCTCCTCAATGG | Start: 414 |
| Tm | 60.034 | 60.036 | End: 526 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | AGGCTTGAAGGGCTTGATCC | CCACTGAGTAACGCCGTTCT | Start: 414 |
| Tm | 60.034 | 60.039 | End: 584 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
|---|
| Sequence | TCGAGGTCAAATGGAAGGGC | CCACTGAGTAACGCCGTTCT | Start: 469 |
| Tm | 60.035 | 60.039 | End: 584 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
|---|
| Sequence | GGTCTGTTGCCATGGAGGAA | CAGAGCTGAGTTGCCTTCGA | Start: 2235 |
| Tm | 59.962 | 60.038 | End: 2367 |
| Size | 20 | 20 | 133 |
(GCA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAGCAATAGTTGAGGCGA | TCCCACGACACAAGACTTCG | Start: 3267 |
| Tm | 60.036 | 59.968 | End: 3391 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
|---|
| Sequence | AGGCTTGAAGGGCTTGATCC | ACAGCCGTTCTCCTCAATGG | Start: 414 |
| Tm | 60.034 | 60.036 | End: 526 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | AGGCTTGAAGGGCTTGATCC | CCACTGAGTAACGCCGTTCT | Start: 414 |
| Tm | 60.034 | 60.039 | End: 584 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
|---|
| Sequence | TCGAGGTCAAATGGAAGGGC | CCACTGAGTAACGCCGTTCT | Start: 469 |
| Tm | 60.035 | 60.039 | End: 584 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
|---|
| Sequence | GGTCTGTTGCCATGGAGGAA | CAGAGCTGAGTTGCCTTCGA | Start: 2235 |
| Tm | 59.962 | 60.038 | End: 2367 |
| Size | 20 | 20 | 133 |
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