ssr_primer
Primer Details for TRINITY_DN9985_c0_g1_i2 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTTTTTCCGTCCTCCAGG | TCGACGCAGAAACCGAGAAA | Start: 543 |
| Tm | 59.89 | 59.969 | End: 671 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
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| Sequence | TGGTTTTTCCGTCCTCCAGG | TTCGACGCAGAAACCGAGAA | Start: 543 |
| Tm | 59.89 | 59.969 | End: 672 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
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| Sequence | TGGTTTTTCCGTCCTCCAGG | TTTCGACGCAGAAACCGAGA | Start: 543 |
| Tm | 59.89 | 59.969 | End: 673 |
| Size | 20 | 20 | 131 |
| Primer 4 details |
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| Sequence | CCCACTCCGACGATGAACAA | AGGATTGGAATGGGAGCGTG | Start: 73 |
| Tm | 60.038 | 60.107 | End: 250 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
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| Sequence | CCCACTCCGACGATGAACAA | ATGGCACGTGGATGAGAGAC | Start: 73 |
| Tm | 60.038 | 59.823 | End: 221 |
| Size | 20 | 20 | 149 |
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