ssr_primer
Primer Details for TRINITY_DN9983_c0_g1_i7 |
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| (TA)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGACCAAGAAAAGCCACGC | GGGCAGCCTGGATTTACCTT | Start: 817 |
| Tm | 60.04 | 60.033 | End: 943 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
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| Sequence | ACAGCTCAAGAGAAACGGGG | GCGTGGCTTTTCTTGGTCTG | Start: 655 |
| Tm | 59.965 | 60.04 | End: 836 |
| Size | 20 | 20 | 182 |
| Primer 3 details |
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| Sequence | ACTGGAAAGAGCACGCATCA | CCAACCTTGATCCGGATGCT | Start: 1352 |
| Tm | 59.965 | 60.107 | End: 1500 |
| Size | 20 | 20 | 149 |
| Primer 4 details |
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| Sequence | ACAGCTCAAGAGAAACGGGG | TTGGTCTGGACACCCCATTG | Start: 655 |
| Tm | 59.965 | 59.888 | End: 824 |
| Size | 20 | 20 | 170 |
| Primer 5 details |
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| Sequence | CTTCTGAGACTCGGGGCAAG | GCGTGGCTTTTCTTGGTCTG | Start: 623 |
| Tm | 60.108 | 60.04 | End: 836 |
| Size | 20 | 20 | 214 |
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