ssr_primer
Primer Details for TRINITY_DN9919_c0_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGCAGCTTCTCTTGGCAT | AACACGATGGCTCCAACCAA | Start: 861 |
| Tm | 60.033 | 60.179 | End: 995 |
| Size | 20 | 20 | 135 |
| Primer 2 details |
|---|
| Sequence | AAGAAGAGCCATGGCAGCTT | AACACGATGGCTCCAACCAA | Start: 851 |
| Tm | 59.961 | 60.179 | End: 995 |
| Size | 20 | 20 | 145 |
| Primer 3 details |
|---|
| Sequence | AGTTGGGTTCGGAGTGCATC | TGGTTTCCATGCCTGCATCT | Start: 177 |
| Tm | 60.322 | 59.96 | End: 356 |
| Size | 20 | 20 | 180 |
| Primer 4 details |
|---|
| Sequence | GAGTTGGGTTCGGAGTGCAT | TGGTTTCCATGCCTGCATCT | Start: 176 |
| Tm | 60.322 | 59.96 | End: 356 |
| Size | 20 | 20 | 181 |
| Primer 5 details |
|---|
| Sequence | CGCAGGCTCGAATTCTTTCC | AGAGATCAGCCTCAGCCTCA | Start: 747 |
| Tm | 59.625 | 60.032 | End: 943 |
| Size | 20 | 20 | 197 |
(GAGGCT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGCAGCTTCTCTTGGCAT | AACACGATGGCTCCAACCAA | Start: 861 |
| Tm | 60.033 | 60.179 | End: 995 |
| Size | 20 | 20 | 135 |
| Primer 2 details |
|---|
| Sequence | AAGAAGAGCCATGGCAGCTT | AACACGATGGCTCCAACCAA | Start: 851 |
| Tm | 59.961 | 60.179 | End: 995 |
| Size | 20 | 20 | 145 |
| Primer 3 details |
|---|
| Sequence | AGTTGGGTTCGGAGTGCATC | TGGTTTCCATGCCTGCATCT | Start: 177 |
| Tm | 60.322 | 59.96 | End: 356 |
| Size | 20 | 20 | 180 |
| Primer 4 details |
|---|
| Sequence | GAGTTGGGTTCGGAGTGCAT | TGGTTTCCATGCCTGCATCT | Start: 176 |
| Tm | 60.322 | 59.96 | End: 356 |
| Size | 20 | 20 | 181 |
| Primer 5 details |
|---|
| Sequence | CGCAGGCTCGAATTCTTTCC | AGAGATCAGCCTCAGCCTCA | Start: 747 |
| Tm | 59.625 | 60.032 | End: 943 |
| Size | 20 | 20 | 197 |
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