ssr_primer
Primer Details for TRINITY_DN9860_c0_g1_i4 |
|---|
| (TCTCT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGGATCCGAACACGATGCG | AAGGTCTCGGCTCTTTCTGC | Start: 1491 |
| Tm | 59.97 | 60.037 | End: 1668 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | GGAAAGGCGTCTGTACTGCT | TGACCTTTGCCTGCTGTTCA | Start: 702 |
| Tm | 60.037 | 60.107 | End: 934 |
| Size | 20 | 20 | 233 |
| Primer 3 details |
|---|
| Sequence | AACTAGCTCCGACGCTTCTG | AGCAGTACAGACGCCTTTCC | Start: 484 |
| Tm | 59.829 | 60.037 | End: 721 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | CAGAACTAGCTCCGACGCTT | AGCAGTACAGACGCCTTTCC | Start: 481 |
| Tm | 59.829 | 60.037 | End: 721 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | TAGGATCCGAACACGATGCG | CTCCAACACTGCCTGTCCAA | Start: 1491 |
| Tm | 59.97 | 60.179 | End: 1768 |
| Size | 20 | 20 | 278 |
(A)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGGATCCGAACACGATGCG | AAGGTCTCGGCTCTTTCTGC | Start: 1491 |
| Tm | 59.97 | 60.037 | End: 1668 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | GGAAAGGCGTCTGTACTGCT | TGACCTTTGCCTGCTGTTCA | Start: 702 |
| Tm | 60.037 | 60.107 | End: 934 |
| Size | 20 | 20 | 233 |
| Primer 3 details |
|---|
| Sequence | AACTAGCTCCGACGCTTCTG | AGCAGTACAGACGCCTTTCC | Start: 484 |
| Tm | 59.829 | 60.037 | End: 721 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | CAGAACTAGCTCCGACGCTT | AGCAGTACAGACGCCTTTCC | Start: 481 |
| Tm | 59.829 | 60.037 | End: 721 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | TAGGATCCGAACACGATGCG | CTCCAACACTGCCTGTCCAA | Start: 1491 |
| Tm | 59.97 | 60.179 | End: 1768 |
| Size | 20 | 20 | 278 |
(AT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGGATCCGAACACGATGCG | AAGGTCTCGGCTCTTTCTGC | Start: 1491 |
| Tm | 59.97 | 60.037 | End: 1668 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | GGAAAGGCGTCTGTACTGCT | TGACCTTTGCCTGCTGTTCA | Start: 702 |
| Tm | 60.037 | 60.107 | End: 934 |
| Size | 20 | 20 | 233 |
| Primer 3 details |
|---|
| Sequence | AACTAGCTCCGACGCTTCTG | AGCAGTACAGACGCCTTTCC | Start: 484 |
| Tm | 59.829 | 60.037 | End: 721 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | CAGAACTAGCTCCGACGCTT | AGCAGTACAGACGCCTTTCC | Start: 481 |
| Tm | 59.829 | 60.037 | End: 721 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | TAGGATCCGAACACGATGCG | CTCCAACACTGCCTGTCCAA | Start: 1491 |
| Tm | 59.97 | 60.179 | End: 1768 |
| Size | 20 | 20 | 278 |
(TA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGGATCCGAACACGATGCG | AAGGTCTCGGCTCTTTCTGC | Start: 1491 |
| Tm | 59.97 | 60.037 | End: 1668 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | GGAAAGGCGTCTGTACTGCT | TGACCTTTGCCTGCTGTTCA | Start: 702 |
| Tm | 60.037 | 60.107 | End: 934 |
| Size | 20 | 20 | 233 |
| Primer 3 details |
|---|
| Sequence | AACTAGCTCCGACGCTTCTG | AGCAGTACAGACGCCTTTCC | Start: 484 |
| Tm | 59.829 | 60.037 | End: 721 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | CAGAACTAGCTCCGACGCTT | AGCAGTACAGACGCCTTTCC | Start: 481 |
| Tm | 59.829 | 60.037 | End: 721 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | TAGGATCCGAACACGATGCG | CTCCAACACTGCCTGTCCAA | Start: 1491 |
| Tm | 59.97 | 60.179 | End: 1768 |
| Size | 20 | 20 | 278 |
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