ssr_primer
Primer Details for TRINITY_DN9758_c0_g1_i4 |
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| (AAT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAATGCGATGAAGGGGCTC | TGTGTGTCGTCTTCTTCCGG | Start: 3257 |
| Tm | 60.179 | 59.968 | End: 3509 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
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| Sequence | AGAATGCGATGAAGGGGCTC | GTGTGTCGTCTTCTTCCGGT | Start: 3257 |
| Tm | 60.179 | 59.968 | End: 3508 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
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| Sequence | AGATGAGTTCGTGGGAAGGC | AGCGACTGAAGAGTTGGGTG | Start: 4057 |
| Tm | 59.749 | 59.966 | End: 4178 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
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| Sequence | TGTGTGCCACGTTAGGTTCA | ACGGGCTCTAAAACAAGGCA | Start: 626 |
| Tm | 59.822 | 59.89 | End: 850 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
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| Sequence | GCAACGGGCTTCAAGTGATG | GAGCCCCTTCATCGCATTCT | Start: 3121 |
| Tm | 60.11 | 60.179 | End: 3276 |
| Size | 20 | 20 | 156 |
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