Primer Details for TRINITY_DN9748_c1_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATCCCAAACTTCAGGAGGCT | TGTCAAACACTTCAACTTTATCCCA | Start: 2 |
| Tm | 59.995 | 59.111 | End: 144 |
| Size | 21 | 25 | 143 |
| Primer 2 details |
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| Sequence | ATCCCAAACTTCAGGAGGCT | TGTCAAACACTTCAACTTTATCCCA | Start: 3 |
| Tm | 58.623 | 59.111 | End: 144 |
| Size | 20 | 25 | 142 |
| Primer 3 details |
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| Sequence | CATCCCAAACTTCAGGAGGC | TGTCAAACACTTCAACTTTATCCCA | Start: 2 |
| Tm | 58.532 | 59.111 | End: 144 |
| Size | 20 | 25 | 143 |
| Primer 4 details |
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| Sequence | GCCATCCCAAACTTCAGGAG | TGTCAAACACTTCAACTTTATCCCA | Start: 0 |
| Tm | 58.532 | 59.111 | End: 144 |
| Size | 20 | 25 | 145 |
| Primer 5 details |
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| Sequence | CATCCCAAACTTCAGGAGGCT | TTGTCAAACACTTCAACTTTATCCCA | Start: 2 |
| Tm | 59.995 | 59.621 | End: 145 |
| Size | 21 | 26 | 144 |
