ssr_primer
Primer Details for TRINITY_DN9748_c0_g1_i6 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTCCACTTCAAATCCCCC | TCTGGTCAAGGGCACATGTC | Start: 860 |
| Tm | 59.962 | 59.963 | End: 1117 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | ACGTCCACTTCAAATCCCCC | CCTTCTGGTCAAGGGCACAT | Start: 860 |
| Tm | 59.962 | 59.961 | End: 1120 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | TCAGGAGGCTGGTTCAACAC | TGTACCAAGTTTCTCGCGCT | Start: 333 |
| Tm | 59.891 | 59.967 | End: 504 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | CCAAACTTCAGGAGGCTGGT | TGTACCAAGTTTCTCGCGCT | Start: 326 |
| Tm | 59.889 | 59.967 | End: 504 |
| Size | 20 | 20 | 179 |
| Primer 5 details |
|---|
| Sequence | GACATGTGCCCTTGACCAGA | GAGAAATGCGTCTCGGGGAA | Start: 1098 |
| Tm | 59.963 | 60.108 | End: 1284 |
| Size | 20 | 20 | 187 |
(AT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTCCACTTCAAATCCCCC | TCTGGTCAAGGGCACATGTC | Start: 860 |
| Tm | 59.962 | 59.963 | End: 1117 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | ACGTCCACTTCAAATCCCCC | CCTTCTGGTCAAGGGCACAT | Start: 860 |
| Tm | 59.962 | 59.961 | End: 1120 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | TCAGGAGGCTGGTTCAACAC | TGTACCAAGTTTCTCGCGCT | Start: 333 |
| Tm | 59.891 | 59.967 | End: 504 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | CCAAACTTCAGGAGGCTGGT | TGTACCAAGTTTCTCGCGCT | Start: 326 |
| Tm | 59.889 | 59.967 | End: 504 |
| Size | 20 | 20 | 179 |
| Primer 5 details |
|---|
| Sequence | GACATGTGCCCTTGACCAGA | GAGAAATGCGTCTCGGGGAA | Start: 1098 |
| Tm | 59.963 | 60.108 | End: 1284 |
| Size | 20 | 20 | 187 |
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