ssr_primer
Primer Details for TRINITY_DN9507_c0_g2_i1 |
|---|
| (A)14cacaaaactctac(AAT)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGTGTGAGCGTTTTGGGTG | TTCAACTCCTCTTGGACCGC | Start: 1952 |
| Tm | 59.971 | 59.965 | End: 2085 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
|---|
| Sequence | GGTTTAGGGTTCCGGTGGAG | AGACTCGGCCTTATGACCCT | Start: 1331 |
| Tm | 60.036 | 60.032 | End: 1558 |
| Size | 20 | 20 | 228 |
| Primer 3 details |
|---|
| Sequence | TCACAAATCGGAACTGCGGA | TTGACCTTGCCGATGTCGAA | Start: 794 |
| Tm | 59.966 | 59.966 | End: 931 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | TCACAAATCGGAACTGCGGA | ACCTTGCCGATGTCGAAGTT | Start: 794 |
| Tm | 59.966 | 59.965 | End: 928 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | GAGGACTTGGTCACGCATCA | AGACTCGGCCTTATGACCCT | Start: 1434 |
| Tm | 60.037 | 60.032 | End: 1558 |
| Size | 20 | 20 | 125 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGTGTGAGCGTTTTGGGTG | TTCAACTCCTCTTGGACCGC | Start: 1952 |
| Tm | 59.971 | 59.965 | End: 2085 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
|---|
| Sequence | GGTTTAGGGTTCCGGTGGAG | AGACTCGGCCTTATGACCCT | Start: 1331 |
| Tm | 60.036 | 60.032 | End: 1558 |
| Size | 20 | 20 | 228 |
| Primer 3 details |
|---|
| Sequence | TCACAAATCGGAACTGCGGA | TTGACCTTGCCGATGTCGAA | Start: 794 |
| Tm | 59.966 | 59.966 | End: 931 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | TCACAAATCGGAACTGCGGA | ACCTTGCCGATGTCGAAGTT | Start: 794 |
| Tm | 59.966 | 59.965 | End: 928 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | GAGGACTTGGTCACGCATCA | AGACTCGGCCTTATGACCCT | Start: 1434 |
| Tm | 60.037 | 60.032 | End: 1558 |
| Size | 20 | 20 | 125 |
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