ssr_primer
Primer Details for TRINITY_DN9464_c0_g1_i4 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCAGGAGATGCTGATGGA | ATGCAAAGCCACCAATGCTG | Start: 2122 |
| Tm | 60.031 | 60.037 | End: 2245 |
| Size | 20 | 20 | 124 |
| Primer 2 details |
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| Sequence | CAGCATTGGTGGCTTTGCAT | CTCACAGGGCCACAGTCAAT | Start: 2226 |
| Tm | 60.037 | 59.963 | End: 2488 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
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| Sequence | TACAGGGCATTTGCTCCTCG | TGGGTAGTTTTCGGGTTCGG | Start: 1545 |
| Tm | 60.108 | 59.965 | End: 1804 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
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| Sequence | CAGCATTGGTGGCTTTGCAT | TGGGAGGTGAAGCAAAGCAA | Start: 2226 |
| Tm | 60.037 | 60.106 | End: 2420 |
| Size | 20 | 20 | 195 |
| Primer 5 details |
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| Sequence | CCAAGTCCAGTGCTGACCAT | TGGGAGGTGAAGCAAAGCAA | Start: 2186 |
| Tm | 59.963 | 60.106 | End: 2420 |
| Size | 20 | 20 | 235 |
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