ssr_primer
Primer Details for TRINITY_DN9431_c0_g1_i4 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGTAACACCCGATGCACA | AACTCCAAAGCATAGCCGCT | Start: 1073 |
| Tm | 60.037 | 60.035 | End: 1176 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
|---|
| Sequence | CCGCTTGTTGATCTTCTGCG | ACAGGCAGCATCGATATCGG | Start: 482 |
| Tm | 59.904 | 60.038 | End: 649 |
| Size | 20 | 20 | 168 |
| Primer 3 details |
|---|
| Sequence | CATGGTGCATGCCCAGATTG | AAACGTTTGTGCATCGGGTG | Start: 847 |
| Tm | 59.896 | 59.97 | End: 1099 |
| Size | 20 | 20 | 253 |
| Primer 4 details |
|---|
| Sequence | CACCCGATGCACAAACGTTT | CCCACTCCGTAAATACCCCG | Start: 1080 |
| Tm | 59.97 | 59.895 | End: 1336 |
| Size | 20 | 20 | 257 |
| Primer 5 details |
|---|
| Sequence | CACCCAATTACCAGGCCAGT | CGCAGAAGATCAACAAGCGG | Start: 252 |
| Tm | 59.96 | 59.904 | End: 501 |
| Size | 20 | 20 | 250 |
(AT)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGTAACACCCGATGCACA | AACTCCAAAGCATAGCCGCT | Start: 1073 |
| Tm | 60.037 | 60.035 | End: 1176 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
|---|
| Sequence | CCGCTTGTTGATCTTCTGCG | ACAGGCAGCATCGATATCGG | Start: 482 |
| Tm | 59.904 | 60.038 | End: 649 |
| Size | 20 | 20 | 168 |
| Primer 3 details |
|---|
| Sequence | CATGGTGCATGCCCAGATTG | AAACGTTTGTGCATCGGGTG | Start: 847 |
| Tm | 59.896 | 59.97 | End: 1099 |
| Size | 20 | 20 | 253 |
| Primer 4 details |
|---|
| Sequence | CACCCGATGCACAAACGTTT | CCCACTCCGTAAATACCCCG | Start: 1080 |
| Tm | 59.97 | 59.895 | End: 1336 |
| Size | 20 | 20 | 257 |
| Primer 5 details |
|---|
| Sequence | CACCCAATTACCAGGCCAGT | CGCAGAAGATCAACAAGCGG | Start: 252 |
| Tm | 59.96 | 59.904 | End: 501 |
| Size | 20 | 20 | 250 |
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