Primer Details for TRINITY_DN9254_c1_g1_i1 |
|---|
| (TTTA)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCTTTTCTCTCTCCCTCA | AAAACAAGTCTCGACAAAAGATAAAAA | Start: 56 |
| Tm | 57.97 | 57.151 | End: 214 |
| Size | 20 | 27 | 159 |
| Primer 2 details |
|---|
| Sequence | TGCCTTTTCTCTCTCCCTCAA | AAAACAAGTCTCGACAAAAGATAAAAA | Start: 56 |
| Tm | 58.661 | 57.151 | End: 214 |
| Size | 21 | 27 | 159 |
| Primer 3 details |
|---|
| Sequence | TTGCCTTTTCTCTCTCCCTCA | AAAACAAGTCTCGACAAAAGATAAAAA | Start: 55 |
| Tm | 58.661 | 57.151 | End: 214 |
| Size | 21 | 27 | 160 |
| Primer 4 details |
|---|
| Sequence | TGCCTTTTCTCTCTCCCTCAAA | AAAACAAGTCTCGACAAAAGATAAAAA | Start: 56 |
| Tm | 59.289 | 57.151 | End: 214 |
| Size | 22 | 27 | 159 |
| Primer 5 details |
|---|
| Sequence | TTGCCTTTTCTCTCTCCCTCAA | AAAACAAGTCTCGACAAAAGATAAAAA | Start: 55 |
| Tm | 59.289 | 57.151 | End: 214 |
| Size | 22 | 27 | 160 |
