ssr_primer
Primer Details for TRINITY_DN9254_c0_g2_i1 |
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| (AAAT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGAACGCCTAATCCGACG | AGAGCTCTCTCCGTCCACAT | Start: 404 |
| Tm | 59.9 | 60.034 | End: 588 |
| Size | 20 | 20 | 185 |
| Primer 2 details |
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| Sequence | ATGTGGACGGAGAGAGCTCT | TCACTAATCGGCTCACTCGC | Start: 569 |
| Tm | 60.034 | 59.899 | End: 788 |
| Size | 20 | 20 | 220 |
| Primer 3 details |
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| Sequence | GGGATTCGGGCATTGTGAGA | AGAGCTCTCTCCGTCCACAT | Start: 389 |
| Tm | 60.107 | 60.034 | End: 588 |
| Size | 20 | 20 | 200 |
| Primer 4 details |
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| Sequence | TGAGAACGCCTAATCCGACG | TGGTGGCCCCAGTCTACATA | Start: 404 |
| Tm | 59.9 | 59.957 | End: 610 |
| Size | 20 | 20 | 207 |
| Primer 5 details |
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| Sequence | CCAGTTGATGTGTGGGGGAA | AGAGCTCTCTCCGTCCACAT | Start: 462 |
| Tm | 59.888 | 60.034 | End: 588 |
| Size | 20 | 20 | 127 |
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