ssr_primer
Primer Details for TRINITY_DN9177_c0_g1_i6 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCACGACCTTGAACCCTG | GAATCGGAGAGCGACGAGAG | Start: 757 |
| Tm | 60.179 | 60.042 | End: 890 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
|---|
| Sequence | CGGTCCAACGGTTGTCAGAT | TTCTCCAACGAGAGGGTTGC | Start: 2239 |
| Tm | 60.32 | 59.965 | End: 2413 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
|---|
| Sequence | AACCACGACCTTGAACCCTG | GAGCGACGAGAGAAAGCCAT | Start: 757 |
| Tm | 60.179 | 60.179 | End: 882 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | CACGACCTTGAACCCTGAGG | GAATCGGAGAGCGACGAGAG | Start: 760 |
| Tm | 60.321 | 60.042 | End: 890 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | CCACGACCTTGAACCCTGAG | GAATCGGAGAGCGACGAGAG | Start: 759 |
| Tm | 60.321 | 60.042 | End: 890 |
| Size | 20 | 20 | 132 |
(A)10cctttttagatgacaataataa(TAG)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCACGACCTTGAACCCTG | GAATCGGAGAGCGACGAGAG | Start: 757 |
| Tm | 60.179 | 60.042 | End: 890 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
|---|
| Sequence | CGGTCCAACGGTTGTCAGAT | TTCTCCAACGAGAGGGTTGC | Start: 2239 |
| Tm | 60.32 | 59.965 | End: 2413 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
|---|
| Sequence | AACCACGACCTTGAACCCTG | GAGCGACGAGAGAAAGCCAT | Start: 757 |
| Tm | 60.179 | 60.179 | End: 882 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | CACGACCTTGAACCCTGAGG | GAATCGGAGAGCGACGAGAG | Start: 760 |
| Tm | 60.321 | 60.042 | End: 890 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | CCACGACCTTGAACCCTGAG | GAATCGGAGAGCGACGAGAG | Start: 759 |
| Tm | 60.321 | 60.042 | End: 890 |
| Size | 20 | 20 | 132 |
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