ssr_primer
Primer Details for TRINITY_DN9131_c0_g1_i3 |
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| (A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCCCTACAGTTTGAAGGCA | ACACCCCAGGCATTTTTCCT | Start: 501 |
| Tm | 60.251 | 59.809 | End: 775 |
| Size | 20 | 20 | 275 |
| Primer 2 details |
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| Sequence | TGCCCCTACAGTTTGAAGGC | ACACCCCAGGCATTTTTCCT | Start: 500 |
| Tm | 60.251 | 59.809 | End: 775 |
| Size | 20 | 20 | 276 |
| Primer 3 details |
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| Sequence | CGGGCTGTGAACTCACTACA | GAAAAGCGGTCATTCCAGCC | Start: 1141 |
| Tm | 59.683 | 59.829 | End: 1412 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
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| Sequence | CGTGTGGTCGGATGGATACC | GCCTTCAAACTGTAGGGGCA | Start: 332 |
| Tm | 60.249 | 60.251 | End: 519 |
| Size | 20 | 20 | 188 |
| Primer 5 details |
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| Sequence | CGTGTGGTCGGATGGATACC | TGCCTTCAAACTGTAGGGGC | Start: 332 |
| Tm | 60.249 | 60.251 | End: 520 |
| Size | 20 | 20 | 189 |
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