ssr_primer
Primer Details for TRINITY_DN9029_c0_g1_i1 |
|---|
| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGCTGTCGGAATTTGGGG | GCGACGACGGAGAGATCATT | Start: 716 |
| Tm | 60.035 | 59.97 | End: 992 |
| Size | 20 | 20 | 277 |
| Primer 2 details |
|---|
| Sequence | AGAGCTGTCGGAATTTGGGG | AAGTGTAATGCGGCACCGTA | Start: 716 |
| Tm | 60.035 | 60.037 | End: 970 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
|---|
| Sequence | AATGATCTCTCCGTCGTCGC | CGTCGCCATCGTTCTGAAAC | Start: 973 |
| Tm | 59.97 | 59.907 | End: 1111 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | TACGGTGCCGCATTACACTT | CGTCGCCATCGTTCTGAAAC | Start: 951 |
| Tm | 60.037 | 59.907 | End: 1111 |
| Size | 20 | 20 | 161 |
| Primer 5 details |
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| Sequence | AATGATCTCTCCGTCGTCGC | CGGCGGGAATACGAAGAAGA | Start: 973 |
| Tm | 59.97 | 59.898 | End: 1163 |
| Size | 20 | 20 | 191 |
(TCA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGCTGTCGGAATTTGGGG | GCGACGACGGAGAGATCATT | Start: 716 |
| Tm | 60.035 | 59.97 | End: 992 |
| Size | 20 | 20 | 277 |
| Primer 2 details |
|---|
| Sequence | AGAGCTGTCGGAATTTGGGG | AAGTGTAATGCGGCACCGTA | Start: 716 |
| Tm | 60.035 | 60.037 | End: 970 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
|---|
| Sequence | AATGATCTCTCCGTCGTCGC | CGTCGCCATCGTTCTGAAAC | Start: 973 |
| Tm | 59.97 | 59.907 | End: 1111 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | TACGGTGCCGCATTACACTT | CGTCGCCATCGTTCTGAAAC | Start: 951 |
| Tm | 60.037 | 59.907 | End: 1111 |
| Size | 20 | 20 | 161 |
| Primer 5 details |
|---|
| Sequence | AATGATCTCTCCGTCGTCGC | CGGCGGGAATACGAAGAAGA | Start: 973 |
| Tm | 59.97 | 59.898 | End: 1163 |
| Size | 20 | 20 | 191 |
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