ssr_primer
Primer Details for TRINITY_DN9025_c0_g1_i2 |
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| (TTC)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACAAAAGCGGTGCGAAACT | CGTTTCAACACTCGCCTTGG | Start: 3 |
| Tm | 59.971 | 60.041 | End: 104 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
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| Sequence | GAGGAGGAACAACGGGGAAG | CTCTTGCACCTGAGCTTCCA | Start: 641 |
| Tm | 60.036 | 59.964 | End: 918 |
| Size | 20 | 20 | 278 |
| Primer 3 details |
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| Sequence | TTTTCCCTCTTGGACTCCGC | CTTCCCCGTTGTTCCTCCTC | Start: 493 |
| Tm | 59.964 | 60.036 | End: 660 |
| Size | 20 | 20 | 168 |
| Primer 4 details |
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| Sequence | GAGGAGGAACAACGGGGAAG | TGCACCTGAGCTTCCAAACA | Start: 641 |
| Tm | 60.036 | 60.107 | End: 914 |
| Size | 20 | 20 | 274 |
| Primer 5 details |
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| Sequence | TTCGAGTGCTTGGGACTGTC | GACTTCTGCAACTCCCCCAA | Start: 932 |
| Tm | 59.966 | 59.89 | End: 1034 |
| Size | 20 | 20 | 103 |
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