ssr_primer
Primer Details for TRINITY_DN8967_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGGGGCTTGCTTAAGGTG | AGGGATAATGGGGGTGGACA | Start: 311 |
| Tm | 59.962 | 59.953 | End: 474 |
| Size | 20 | 20 | 164 |
| Primer 2 details |
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| Sequence | TCTGGGGCTTGCTTAAGGTG | TAATGGGGGTGGACAATCGC | Start: 311 |
| Tm | 59.962 | 60.107 | End: 469 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
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| Sequence | ACACCGACTCCTCCAGAAGA | CACCTTAAGCAAGCCCCAGA | Start: 92 |
| Tm | 59.887 | 59.962 | End: 330 |
| Size | 20 | 20 | 239 |
| Primer 4 details |
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| Sequence | CCGATCTGGGGCTTGCTTAA | AGGGATAATGGGGGTGGACA | Start: 307 |
| Tm | 60.107 | 59.953 | End: 474 |
| Size | 20 | 20 | 168 |
| Primer 5 details |
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| Sequence | TGTCCACCCCCATTATCCCT | GCCACAGCAACACACAAACA | Start: 455 |
| Tm | 59.953 | 60.109 | End: 667 |
| Size | 20 | 20 | 213 |
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