Primer Details for TRINITY_DN890_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTTCATTTCGTGGTGATACATGT | GCCCAGTACTGTGCAACACT | Start: 131 |
| Tm | 58.754 | 60.535 | End: 246 |
| Size | 24 | 20 | 116 |
| Primer 2 details | |||
| Sequence | AGTTCATTTCGTGGTGATACATGT | AGCCCAGTACTGTGCAACAC | Start: 131 |
| Tm | 58.754 | 60.535 | End: 247 |
| Size | 24 | 20 | 117 |
| Primer 3 details | |||
| Sequence | AGTTCATTTCGTGGTGATACATGT | ACAGCCCAGTACTGTGCAAC | Start: 131 |
| Tm | 58.754 | 60.535 | End: 249 |
| Size | 24 | 20 | 119 |
| Primer 4 details | |||
| Sequence | CGGTAAATAGTTCATTTCGTGGTGA | GCCCAGTACTGTGCAACACT | Start: 123 |
| Tm | 59.651 | 60.535 | End: 246 |
| Size | 25 | 20 | 124 |
| Primer 5 details | |||
| Sequence | CGGTAAATAGTTCATTTCGTGGTGA | AGCCCAGTACTGTGCAACAC | Start: 123 |
| Tm | 59.651 | 60.535 | End: 247 |
| Size | 25 | 20 | 125 |
