ssr_primer
Primer Details for TRINITY_DN8721_c0_g1_i2 |
|---|
| (CTT)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGCTTCTCTCACATGGCCA | AAGATCGGCGACCACCATTT | Start: 19 |
| Tm | 60.036 | 60.035 | End: 165 |
| Size | 20 | 20 | 147 |
| Primer 2 details |
|---|
| Sequence | ACATGGCCACCAATTTTGCC | AAGATCGGCGACCACCATTT | Start: 30 |
| Tm | 59.963 | 60.035 | End: 165 |
| Size | 20 | 20 | 136 |
| Primer 3 details |
|---|
| Sequence | AAATGGTGGTCGCCGATCTT | GCTCTCCCGCGTTTTCATTC | Start: 146 |
| Tm | 60.035 | 59.903 | End: 331 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | GAATGAAAACGCGGGAGAGC | ACAATACCTCACTGCGCCAA | Start: 312 |
| Tm | 59.903 | 59.964 | End: 448 |
| Size | 20 | 20 | 137 |
| Primer 5 details |
|---|
| Sequence | TTGGCGCAGTGAGGTATTGT | TTTCTAGGCCGTCGACATCG | Start: 429 |
| Tm | 59.964 | 59.9 | End: 708 |
| Size | 20 | 20 | 280 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGCTTCTCTCACATGGCCA | AAGATCGGCGACCACCATTT | Start: 19 |
| Tm | 60.036 | 60.035 | End: 165 |
| Size | 20 | 20 | 147 |
| Primer 2 details |
|---|
| Sequence | ACATGGCCACCAATTTTGCC | AAGATCGGCGACCACCATTT | Start: 30 |
| Tm | 59.963 | 60.035 | End: 165 |
| Size | 20 | 20 | 136 |
| Primer 3 details |
|---|
| Sequence | AAATGGTGGTCGCCGATCTT | GCTCTCCCGCGTTTTCATTC | Start: 146 |
| Tm | 60.035 | 59.903 | End: 331 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | GAATGAAAACGCGGGAGAGC | ACAATACCTCACTGCGCCAA | Start: 312 |
| Tm | 59.903 | 59.964 | End: 448 |
| Size | 20 | 20 | 137 |
| Primer 5 details |
|---|
| Sequence | TTGGCGCAGTGAGGTATTGT | TTTCTAGGCCGTCGACATCG | Start: 429 |
| Tm | 59.964 | 59.9 | End: 708 |
| Size | 20 | 20 | 280 |
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