Primer Details for TRINITY_DN8452_c0_g1_i2 |
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| (A)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAACCAAAGATTTCAAATGGAAGC | TGTTGCCAAAATGACCGTAGC | Start: 7 |
| Tm | 59.82 | 59.732 | End: 218 |
| Size | 25 | 21 | 212 |
| Primer 2 details |
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| Sequence | CCAACCAAAGATTTCAAATGGAAGC | TGCCAAAATGACCGTAGCTCA | Start: 7 |
| Tm | 59.82 | 60.271 | End: 215 |
| Size | 25 | 21 | 209 |
| Primer 3 details |
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| Sequence | CCAACCAAAGATTTCAAATGGAAGC | TGACCGTAGCTCAAGTGACA | Start: 7 |
| Tm | 59.82 | 58.676 | End: 207 |
| Size | 25 | 20 | 201 |
| Primer 4 details |
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| Sequence | GTGAAGTGGGGTAATCGAACCT | GCCAAACGAAAGATATCATGTCCT | Start: 141 |
| Tm | 60.027 | 59.424 | End: 264 |
| Size | 22 | 24 | 124 |
| Primer 5 details |
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| Sequence | GTGAAGTGGGGTAATCGAACCT | AGCCAAACGAAAGATATCATGTCC | Start: 141 |
| Tm | 60.027 | 59.424 | End: 265 |
| Size | 22 | 24 | 125 |
