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CsExSLDb: Transcriptome based Cucumis sativus Extended Shelf Life Database

ssr_primer

Primer Details for TRINITY_DN8296_c0_g1_i1

(ACA)5aagtttgaagctaatatacaaatatagtataacatcaaacgaattcccccttatacttacaaatgacaaagaattca(AT)6: Primer 1 details
Primer CharacteristicsForward PrimerReverse PrimerProduct Details
SequenceGCTTCTCTTCAACCGGTGGATCCCTCCTCATCCTCCTGTGStart: 1124
Tm59.96560.032End: 1270
Size2020147
Primer 2 details
SequenceCACATAAACCGGTGGAGGCTTCCCTCCTCATCCTCCTGTGStart: 1107
Tm60.03560.032End: 1270
Size2020164
Primer 3 details
SequenceAGGCTTCTCTTCAACCGGTGTCCCTCCTCATCCTCCTGTGStart: 1122
Tm59.96560.032End: 1270
Size2020149
Primer 4 details
SequenceTTGTGGTGTTGGAGGCTTGTTCCACCGGTTGAAGAGAAGCStart: 870
Tm60.03459.965End: 1143
Size2020274
Primer 5 details
SequenceTTGTGGTGTTGGAGGCTTGTAGCCTCCACCGGTTTATGTGStart: 870
Tm60.03460.035End: 1126
Size2020257
(T)12gcttagattcaagtttgcatcttcgtttctaac(A)10: Primer 1 details
Primer CharacteristicsForward PrimerReverse PrimerProduct Details
SequenceGCTTCTCTTCAACCGGTGGATCCCTCCTCATCCTCCTGTGStart: 1124
Tm59.96560.032End: 1270
Size2020147
Primer 2 details
SequenceCACATAAACCGGTGGAGGCTTCCCTCCTCATCCTCCTGTGStart: 1107
Tm60.03560.032End: 1270
Size2020164
Primer 3 details
SequenceAGGCTTCTCTTCAACCGGTGTCCCTCCTCATCCTCCTGTGStart: 1122
Tm59.96560.032End: 1270
Size2020149
Primer 4 details
SequenceTTGTGGTGTTGGAGGCTTGTTCCACCGGTTGAAGAGAAGCStart: 870
Tm60.03459.965End: 1143
Size2020274
Primer 5 details
SequenceTTGTGGTGTTGGAGGCTTGTAGCCTCCACCGGTTTATGTGStart: 870
Tm60.03460.035End: 1126
Size2020257

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