ssr_primer
Primer Details for TRINITY_DN8290_c0_g1_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTTTCCCACCCTTTCGAGA | CGACCACAGCTCCTCAACAT | Start: 234 |
| Tm | 59.966 | 60.037 | End: 486 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
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| Sequence | CTCCCCGATCAAACCCACAA | TCTCGAAAGGGTGGGAAACG | Start: 97 |
| Tm | 59.962 | 59.966 | End: 253 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
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| Sequence | GTCCTCCGGCAGCTAAAAGT | GACCACAGGGAAGCTTCCTC | Start: 1016 |
| Tm | 60.036 | 60.036 | End: 1162 |
| Size | 20 | 20 | 147 |
| Primer 4 details |
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| Sequence | TCCTCCGGCAGCTAAAAGTG | GACCACAGGGAAGCTTCCTC | Start: 1017 |
| Tm | 60.036 | 60.036 | End: 1162 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
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| Sequence | TGGAGAAGAGGTGGGTCGAT | ACAACGACTTCCTCTGCCAG | Start: 852 |
| Tm | 59.959 | 59.966 | End: 1100 |
| Size | 20 | 20 | 249 |
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