ssr_primer
Primer Details for TRINITY_DN8228_c0_g1_i4 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGCTTGTGCAGTGTCCTAT | GCATGAAGGGGAAGCAGGAT | Start: 730 |
| Tm | 60.035 | 60.106 | End: 858 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
|---|
| Sequence | CAATACTGTTGCCGGTGCTG | ATAGGACACTGCACAAGCCC | Start: 474 |
| Tm | 59.831 | 60.035 | End: 749 |
| Size | 20 | 20 | 276 |
| Primer 3 details |
|---|
| Sequence | ATTTCTGTGACCCCGAGCTG | CAGCACCGGCAACAGTATTG | Start: 320 |
| Tm | 60.036 | 59.831 | End: 493 |
| Size | 20 | 20 | 174 |
| Primer 4 details |
|---|
| Sequence | TGATTTCTGTGACCCCGAGC | CAGCACCGGCAACAGTATTG | Start: 318 |
| Tm | 60.036 | 59.831 | End: 493 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
|---|
| Sequence | CAATACTGTTGCCGGTGCTG | TCGAAAACGAAGCCGATGGA | Start: 474 |
| Tm | 59.831 | 60.038 | End: 643 |
| Size | 20 | 20 | 170 |
(AGA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGCTTGTGCAGTGTCCTAT | GCATGAAGGGGAAGCAGGAT | Start: 730 |
| Tm | 60.035 | 60.106 | End: 858 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
|---|
| Sequence | CAATACTGTTGCCGGTGCTG | ATAGGACACTGCACAAGCCC | Start: 474 |
| Tm | 59.831 | 60.035 | End: 749 |
| Size | 20 | 20 | 276 |
| Primer 3 details |
|---|
| Sequence | ATTTCTGTGACCCCGAGCTG | CAGCACCGGCAACAGTATTG | Start: 320 |
| Tm | 60.036 | 59.831 | End: 493 |
| Size | 20 | 20 | 174 |
| Primer 4 details |
|---|
| Sequence | TGATTTCTGTGACCCCGAGC | CAGCACCGGCAACAGTATTG | Start: 318 |
| Tm | 60.036 | 59.831 | End: 493 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
|---|
| Sequence | CAATACTGTTGCCGGTGCTG | TCGAAAACGAAGCCGATGGA | Start: 474 |
| Tm | 59.831 | 60.038 | End: 643 |
| Size | 20 | 20 | 170 |
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