ssr_primer
Primer Details for TRINITY_DN8227_c0_g1_i1 |
|---|
| (CAC)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCACGTGCTTACGATGAAG | TCGCCATCTTCGTCGTTGAT | Start: 535 |
| Tm | 59.976 | 59.827 | End: 807 |
| Size | 20 | 20 | 273 |
| Primer 2 details |
|---|
| Sequence | CATTTCCTCCGCGTCCTCTT | CACGTATCGGGTCACGGATT | Start: 212 |
| Tm | 60.108 | 59.898 | End: 491 |
| Size | 20 | 20 | 280 |
| Primer 3 details |
|---|
| Sequence | CATTTCCTCCGCGTCCTCTT | GATTTCAGCCGCCCATTTCC | Start: 212 |
| Tm | 60.108 | 59.897 | End: 475 |
| Size | 20 | 20 | 264 |
| Primer 4 details |
|---|
| Sequence | CATTTCCTCCGCGTCCTCTT | GGATTTCAGCCGCCCATTTC | Start: 212 |
| Tm | 60.108 | 59.897 | End: 476 |
| Size | 20 | 20 | 265 |
| Primer 5 details |
|---|
| Sequence | CCTCTCTTTCCACCACGACC | TAGTAGTGGTAGAGGCGGGG | Start: 193 |
| Tm | 60.037 | 59.816 | End: 353 |
| Size | 20 | 20 | 161 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCACGTGCTTACGATGAAG | TCGCCATCTTCGTCGTTGAT | Start: 535 |
| Tm | 59.976 | 59.827 | End: 807 |
| Size | 20 | 20 | 273 |
| Primer 2 details |
|---|
| Sequence | CATTTCCTCCGCGTCCTCTT | CACGTATCGGGTCACGGATT | Start: 212 |
| Tm | 60.108 | 59.898 | End: 491 |
| Size | 20 | 20 | 280 |
| Primer 3 details |
|---|
| Sequence | CATTTCCTCCGCGTCCTCTT | GATTTCAGCCGCCCATTTCC | Start: 212 |
| Tm | 60.108 | 59.897 | End: 475 |
| Size | 20 | 20 | 264 |
| Primer 4 details |
|---|
| Sequence | CATTTCCTCCGCGTCCTCTT | GGATTTCAGCCGCCCATTTC | Start: 212 |
| Tm | 60.108 | 59.897 | End: 476 |
| Size | 20 | 20 | 265 |
| Primer 5 details |
|---|
| Sequence | CCTCTCTTTCCACCACGACC | TAGTAGTGGTAGAGGCGGGG | Start: 193 |
| Tm | 60.037 | 59.816 | End: 353 |
| Size | 20 | 20 | 161 |
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