ssr_primer
Primer Details for TRINITY_DN8203_c0_g1_i1 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGCGGACCACAAAAGGATA | TCTCTAAGGCCGGTGATGGA | Start: 65 |
| Tm | 60.035 | 60.033 | End: 299 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
|---|
| Sequence | AAGCAGAAGCAGAAGCAGGT | AACCGCGTCTATGGCCTATG | Start: 856 |
| Tm | 59.89 | 59.966 | End: 1031 |
| Size | 20 | 20 | 176 |
| Primer 3 details |
|---|
| Sequence | AAGCAGAAGCAGAAGCAGGT | AAACCCCATAAACCGCGTCT | Start: 856 |
| Tm | 59.89 | 59.963 | End: 1041 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | AAGCAGAAGCAGAAGCAGGT | TGGCCTATGGGATTGCACTG | Start: 856 |
| Tm | 59.89 | 60.107 | End: 1020 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | AGTGGGAGAGTCACATGGGA | TGGCCTATGGGATTGCACTG | Start: 748 |
| Tm | 59.883 | 60.107 | End: 1020 |
| Size | 20 | 20 | 273 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGCGGACCACAAAAGGATA | TCTCTAAGGCCGGTGATGGA | Start: 65 |
| Tm | 60.035 | 60.033 | End: 299 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
|---|
| Sequence | AAGCAGAAGCAGAAGCAGGT | AACCGCGTCTATGGCCTATG | Start: 856 |
| Tm | 59.89 | 59.966 | End: 1031 |
| Size | 20 | 20 | 176 |
| Primer 3 details |
|---|
| Sequence | AAGCAGAAGCAGAAGCAGGT | AAACCCCATAAACCGCGTCT | Start: 856 |
| Tm | 59.89 | 59.963 | End: 1041 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | AAGCAGAAGCAGAAGCAGGT | TGGCCTATGGGATTGCACTG | Start: 856 |
| Tm | 59.89 | 60.107 | End: 1020 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | AGTGGGAGAGTCACATGGGA | TGGCCTATGGGATTGCACTG | Start: 748 |
| Tm | 59.883 | 60.107 | End: 1020 |
| Size | 20 | 20 | 273 |
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