Primer Details for TRINITY_DN8172_c0_g2_i1 | |||
|---|---|---|---|
| (T)17: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTCAAATGTTCAAGTTCACAA | AACGCACTAGTCACTCATTGA | Start: 118 |
| Tm | 57.447 | 57.339 | End: 235 |
| Size | 23 | 21 | 118 |
| Primer 2 details | |||
| Sequence | TCGTCAAATGTTCAAGTTCACAA | TCTCTAACGCACTAGTCACTCA | Start: 118 |
| Tm | 57.447 | 58.328 | End: 240 |
| Size | 23 | 22 | 123 |
| Primer 3 details | |||
| Sequence | TGAGGTGGAGTTTGATTCAAAATGT | AACGCACTAGTCACTCATTGA | Start: 66 |
| Tm | 59.402 | 57.339 | End: 235 |
| Size | 25 | 21 | 170 |
| Primer 4 details | |||
| Sequence | TGAGGTGGAGTTTGATTCAAAATGT | TCTCTAACGCACTAGTCACTCA | Start: 66 |
| Tm | 59.402 | 58.328 | End: 240 |
| Size | 25 | 22 | 175 |
| Primer 5 details | |||
| Sequence | ATCGTCAAATGTTCAAGTTCACA | AACGCACTAGTCACTCATTGA | Start: 117 |
| Tm | 57.25 | 57.339 | End: 235 |
| Size | 23 | 21 | 119 |
