ssr_primer
Primer Details for TRINITY_DN8136_c0_g1_i3 |
|---|
| (T)10cctttcattcttctcttatctaaatcatca(TCT)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGTGGTGGCTAGTGAAGTT | GCAAGAGTGTCATTTGGCCG | Start: 255 |
| Tm | 59.965 | 60.11 | End: 402 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | AGAGGTTGTATTGGCCGGTG | GCAAGAGTGTCATTTGGCCG | Start: 240 |
| Tm | 60.035 | 60.11 | End: 402 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | CGGCCAAATGACACTCTTGC | AGCCCGCAACACCTTCATAA | Start: 383 |
| Tm | 60.11 | 59.962 | End: 551 |
| Size | 20 | 20 | 169 |
| Primer 4 details |
|---|
| Sequence | CGGCCAAATGACACTCTTGC | TTAGCCCGCAACACCTTCAT | Start: 383 |
| Tm | 60.11 | 59.962 | End: 553 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | CGGTGGTGGCTAGTGAAGTT | GCTTGACACGAGTAAACCGC | Start: 255 |
| Tm | 59.965 | 59.837 | End: 377 |
| Size | 20 | 20 | 123 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGTGGTGGCTAGTGAAGTT | GCAAGAGTGTCATTTGGCCG | Start: 255 |
| Tm | 59.965 | 60.11 | End: 402 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | AGAGGTTGTATTGGCCGGTG | GCAAGAGTGTCATTTGGCCG | Start: 240 |
| Tm | 60.035 | 60.11 | End: 402 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | CGGCCAAATGACACTCTTGC | AGCCCGCAACACCTTCATAA | Start: 383 |
| Tm | 60.11 | 59.962 | End: 551 |
| Size | 20 | 20 | 169 |
| Primer 4 details |
|---|
| Sequence | CGGCCAAATGACACTCTTGC | TTAGCCCGCAACACCTTCAT | Start: 383 |
| Tm | 60.11 | 59.962 | End: 553 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | CGGTGGTGGCTAGTGAAGTT | GCTTGACACGAGTAAACCGC | Start: 255 |
| Tm | 59.965 | 59.837 | End: 377 |
| Size | 20 | 20 | 123 |
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