ssr_primer
Primer Details for TRINITY_DN8118_c0_g1_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCAAGCTCTTCGATGGGGG | CCCCCACCACCATCATCTTC | Start: 814 |
| Tm | 59.747 | 60.107 | End: 955 |
| Size | 20 | 20 | 142 |
| Primer 2 details |
|---|
| Sequence | CCCTGGTGAACTCCATGGAC | TCCCGGATGAAACTGAAGCA | Start: 532 |
| Tm | 60.035 | 59.31 | End: 680 |
| Size | 20 | 20 | 149 |
| Primer 3 details |
|---|
| Sequence | TTCAAGCTCTTCGATGGGGG | TTTTCCCTTCTGACCACCCC | Start: 814 |
| Tm | 59.747 | 59.519 | End: 971 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | TCCCTGGTGAACTCCATGGA | TCCCGGATGAAACTGAAGCA | Start: 531 |
| Tm | 60.179 | 59.31 | End: 680 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | TTCAAGCTCTTCGATGGGGG | CCCCACCACCATCATCTTCA | Start: 814 |
| Tm | 59.747 | 59.375 | End: 954 |
| Size | 20 | 20 | 141 |
(AGA)7atgaacaatggaaagaaaatggcagacttttacaaacttttgtctctctattattataatatcttattgaccaaactcctttactttattttattccat(TTTTC)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCAAGCTCTTCGATGGGGG | CCCCCACCACCATCATCTTC | Start: 814 |
| Tm | 59.747 | 60.107 | End: 955 |
| Size | 20 | 20 | 142 |
| Primer 2 details |
|---|
| Sequence | CCCTGGTGAACTCCATGGAC | TCCCGGATGAAACTGAAGCA | Start: 532 |
| Tm | 60.035 | 59.31 | End: 680 |
| Size | 20 | 20 | 149 |
| Primer 3 details |
|---|
| Sequence | TTCAAGCTCTTCGATGGGGG | TTTTCCCTTCTGACCACCCC | Start: 814 |
| Tm | 59.747 | 59.519 | End: 971 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | TCCCTGGTGAACTCCATGGA | TCCCGGATGAAACTGAAGCA | Start: 531 |
| Tm | 60.179 | 59.31 | End: 680 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | TTCAAGCTCTTCGATGGGGG | CCCCACCACCATCATCTTCA | Start: 814 |
| Tm | 59.747 | 59.375 | End: 954 |
| Size | 20 | 20 | 141 |
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