ssr_primer
Primer Details for TRINITY_DN8025_c0_g1_i4 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACGGCTCTTTCTCCTCCCA | GAAGAGCGGGAACAAGTGGA | Start: 929 |
| Tm | 59.959 | 59.965 | End: 1149 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | TACGGCTCTTTCTCCTCCCA | AGCGGGAACAAGTGGATCTG | Start: 929 |
| Tm | 59.959 | 60.036 | End: 1145 |
| Size | 20 | 20 | 217 |
| Primer 3 details |
|---|
| Sequence | TACGGCTCTTTCTCCTCCCA | GTGCAGAAGGTGTTTGCTCG | Start: 929 |
| Tm | 59.959 | 60.041 | End: 1100 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | TCCACTTGTTCCCGCTCTTC | GATCTTCATCGCCGTCCAGT | Start: 1130 |
| Tm | 59.965 | 59.897 | End: 1285 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
|---|
| Sequence | CTCCAATCGCTACGGCTCTT | GAAGAGCGGGAACAAGTGGA | Start: 919 |
| Tm | 59.897 | 59.965 | End: 1149 |
| Size | 20 | 20 | 231 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACGGCTCTTTCTCCTCCCA | GAAGAGCGGGAACAAGTGGA | Start: 929 |
| Tm | 59.959 | 59.965 | End: 1149 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | TACGGCTCTTTCTCCTCCCA | AGCGGGAACAAGTGGATCTG | Start: 929 |
| Tm | 59.959 | 60.036 | End: 1145 |
| Size | 20 | 20 | 217 |
| Primer 3 details |
|---|
| Sequence | TACGGCTCTTTCTCCTCCCA | GTGCAGAAGGTGTTTGCTCG | Start: 929 |
| Tm | 59.959 | 60.041 | End: 1100 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | TCCACTTGTTCCCGCTCTTC | GATCTTCATCGCCGTCCAGT | Start: 1130 |
| Tm | 59.965 | 59.897 | End: 1285 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
|---|
| Sequence | CTCCAATCGCTACGGCTCTT | GAAGAGCGGGAACAAGTGGA | Start: 919 |
| Tm | 59.897 | 59.965 | End: 1149 |
| Size | 20 | 20 | 231 |
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