Primer Details for TRINITY_DN8020_c0_g1_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTGAAATTTTCATGTGGGTGT | ACAGGCATGATCATATTCTATGGACT | Start: 156 |
| Tm | 57.132 | 59.785 | End: 276 |
| Size | 23 | 26 | 121 |
| Primer 2 details |
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| Sequence | TCTTGAAATTTTCATGTGGGTGT | ACAGGCATGATCATATTCTATGGAC | Start: 156 |
| Tm | 57.132 | 58.587 | End: 276 |
| Size | 23 | 25 | 121 |
| Primer 3 details |
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| Sequence | TCTTGAAATTTTCATGTGGGTGTT | ACAGGCATGATCATATTCTATGGACT | Start: 156 |
| Tm | 57.767 | 59.785 | End: 276 |
| Size | 24 | 26 | 121 |
| Primer 4 details |
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| Sequence | TTCTTGAAATTTTCATGTGGGTGT | ACAGGCATGATCATATTCTATGGACT | Start: 155 |
| Tm | 57.767 | 59.785 | End: 276 |
| Size | 24 | 26 | 122 |
| Primer 5 details |
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| Sequence | TCTTGAAATTTTCATGTGGGTGT | ACAGGCATGATCATATTCTATGGA | Start: 156 |
| Tm | 57.132 | 57.368 | End: 276 |
| Size | 23 | 24 | 121 |
