ssr_primer
Primer Details for TRINITY_DN7977_c0_g1_i3 |
|---|
| (TCA)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCACAGAAGCAACCTCGA | GGGAGCCGTGAGAGTGAAAT | Start: 10 |
| Tm | 59.966 | 59.749 | End: 165 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | ATTTCACTCTCACGGCTCCC | TTCCGAACTCCGATTCCGAC | Start: 146 |
| Tm | 59.749 | 59.828 | End: 323 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
|---|
| Sequence | GGTCACAGAAGCAACCTCGA | CCGCCTCCGATTCAAAGGAA | Start: 10 |
| Tm | 59.966 | 60.392 | End: 285 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TGAGCACACTTTGAAGGGCA | TCGGGTTCAAATCTCCACCC | Start: 1436 |
| Tm | 60.107 | 59.673 | End: 1561 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | TGGTCACAGAAGCAACCTCG | GGGAGCCGTGAGAGTGAAAT | Start: 9 |
| Tm | 60.25 | 59.749 | End: 165 |
| Size | 20 | 20 | 157 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCACAGAAGCAACCTCGA | GGGAGCCGTGAGAGTGAAAT | Start: 10 |
| Tm | 59.966 | 59.749 | End: 165 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | ATTTCACTCTCACGGCTCCC | TTCCGAACTCCGATTCCGAC | Start: 146 |
| Tm | 59.749 | 59.828 | End: 323 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
|---|
| Sequence | GGTCACAGAAGCAACCTCGA | CCGCCTCCGATTCAAAGGAA | Start: 10 |
| Tm | 59.966 | 60.392 | End: 285 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TGAGCACACTTTGAAGGGCA | TCGGGTTCAAATCTCCACCC | Start: 1436 |
| Tm | 60.107 | 59.673 | End: 1561 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | TGGTCACAGAAGCAACCTCG | GGGAGCCGTGAGAGTGAAAT | Start: 9 |
| Tm | 60.25 | 59.749 | End: 165 |
| Size | 20 | 20 | 157 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCACAGAAGCAACCTCGA | GGGAGCCGTGAGAGTGAAAT | Start: 10 |
| Tm | 59.966 | 59.749 | End: 165 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | ATTTCACTCTCACGGCTCCC | TTCCGAACTCCGATTCCGAC | Start: 146 |
| Tm | 59.749 | 59.828 | End: 323 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
|---|
| Sequence | GGTCACAGAAGCAACCTCGA | CCGCCTCCGATTCAAAGGAA | Start: 10 |
| Tm | 59.966 | 60.392 | End: 285 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TGAGCACACTTTGAAGGGCA | TCGGGTTCAAATCTCCACCC | Start: 1436 |
| Tm | 60.107 | 59.673 | End: 1561 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | TGGTCACAGAAGCAACCTCG | GGGAGCCGTGAGAGTGAAAT | Start: 9 |
| Tm | 60.25 | 59.749 | End: 165 |
| Size | 20 | 20 | 157 |
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