ssr_primer
Primer Details for TRINITY_DN7959_c0_g1_i2 |
|---|
| (TCG)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGGCGATGGTATTAGTGGA | GCTTCTTTGCCGGTTTTCGT | Start: 1079 |
| Tm | 59.967 | 59.97 | End: 1244 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
|---|
| Sequence | AGCGGCGATGGTATTAGTGG | GCTTCTTTGCCGGTTTTCGT | Start: 1078 |
| Tm | 59.966 | 59.97 | End: 1244 |
| Size | 20 | 20 | 167 |
| Primer 3 details |
|---|
| Sequence | CTGCTGGTTTACCCATCGGT | AGGGTTGCAGGGAATGTAGC | Start: 387 |
| Tm | 60.035 | 60.034 | End: 637 |
| Size | 20 | 20 | 251 |
| Primer 4 details |
|---|
| Sequence | TGCTGGTTTACCCATCGGTC | AGGGTTGCAGGGAATGTAGC | Start: 388 |
| Tm | 60.036 | 60.034 | End: 637 |
| Size | 20 | 20 | 250 |
| Primer 5 details |
|---|
| Sequence | GCGGCGATGGTATTAGTGGA | TAACCGACTCCGCCAGTTTC | Start: 1079 |
| Tm | 59.967 | 60.038 | End: 1292 |
| Size | 20 | 20 | 214 |
(T)14atattaaac(AT)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGGCGATGGTATTAGTGGA | GCTTCTTTGCCGGTTTTCGT | Start: 1079 |
| Tm | 59.967 | 59.97 | End: 1244 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
|---|
| Sequence | AGCGGCGATGGTATTAGTGG | GCTTCTTTGCCGGTTTTCGT | Start: 1078 |
| Tm | 59.966 | 59.97 | End: 1244 |
| Size | 20 | 20 | 167 |
| Primer 3 details |
|---|
| Sequence | CTGCTGGTTTACCCATCGGT | AGGGTTGCAGGGAATGTAGC | Start: 387 |
| Tm | 60.035 | 60.034 | End: 637 |
| Size | 20 | 20 | 251 |
| Primer 4 details |
|---|
| Sequence | TGCTGGTTTACCCATCGGTC | AGGGTTGCAGGGAATGTAGC | Start: 388 |
| Tm | 60.036 | 60.034 | End: 637 |
| Size | 20 | 20 | 250 |
| Primer 5 details |
|---|
| Sequence | GCGGCGATGGTATTAGTGGA | TAACCGACTCCGCCAGTTTC | Start: 1079 |
| Tm | 59.967 | 60.038 | End: 1292 |
| Size | 20 | 20 | 214 |
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