ssr_primer
Primer Details for TRINITY_DN7700_c0_g1_i1 |
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| (TCC)8: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTTTTCCTCCCGCCGAAT | AAGGCAAGACATGGAGGTGG | Start: 314 |
| Tm | 59.962 | 59.961 | End: 425 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
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| Sequence | AACTTTTCCTCCCGCCGAAT | CGATCGTTCAGGAGGGAAGG | Start: 314 |
| Tm | 59.962 | 59.897 | End: 441 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
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| Sequence | GGTATGTGGTCACAGTCGGG | TTTCTTCCGCCATAAGCCGT | Start: 1168 |
| Tm | 60.108 | 60.036 | End: 1291 |
| Size | 20 | 20 | 124 |
| Primer 4 details |
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| Sequence | CCACCTCCATGTCTTGCCTT | CAGAAGCAGAAGGAGAGGGC | Start: 406 |
| Tm | 59.961 | 60.108 | End: 626 |
| Size | 20 | 20 | 221 |
| Primer 5 details |
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| Sequence | GGAAGGCGAGAGTGTGAGAG | TTTCTTCCGCCATAAGCCGT | Start: 1186 |
| Tm | 59.828 | 60.036 | End: 1291 |
| Size | 20 | 20 | 106 |
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