Primer Details for TRINITY_DN7649_c0_g1_i1 | |||
|---|---|---|---|
| (T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTACAGAGGATGTGAATGGGA | AGCCCCATGATTCAATACTTTACCT | Start: 144 |
| Tm | 58.55 | 60.104 | End: 308 |
| Size | 22 | 25 | 165 |
| Primer 2 details | |||
| Sequence | TGTACAGAGGATGTGAATGGGA | GCCCCATGATTCAATACTTTACCT | Start: 144 |
| Tm | 58.55 | 58.86 | End: 307 |
| Size | 22 | 24 | 164 |
| Primer 3 details | |||
| Sequence | TGTACAGAGGATGTGAATGGGA | AGCCCCATGATTCAATACTTTACC | Start: 144 |
| Tm | 58.55 | 58.86 | End: 308 |
| Size | 22 | 24 | 165 |
| Primer 4 details | |||
| Sequence | TGTACAGAGGATGTGAATGGGA | GCCCCATGATTCAATACTTTACC | Start: 144 |
| Tm | 58.55 | 57.546 | End: 307 |
| Size | 22 | 23 | 164 |
| Primer 5 details | |||
| Sequence | TTGTACAGAGGATGTGAATGGGA | AGCCCCATGATTCAATACTTTACCT | Start: 143 |
| Tm | 59.156 | 60.104 | End: 308 |
| Size | 23 | 25 | 166 |
