Primer Details for TRINITY_DN7572_c0_g1_i1 |
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| (A)12ggagacagaactttacattaagagaatgaaaaaagaaacaatgttcaaagaagtgaaaaagagcaaaatagaaag(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGAGGGGAGGCTAGGGAGT | ACCTCTCTAACTTACTTTTTGCCCT | Start: 7 |
| Tm | 60.33 | 59.927 | End: 172 |
| Size | 20 | 25 | 166 |
| Primer 2 details |
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| Sequence | TAGAGGGGAGGCTAGGGAGT | CACCTCTCTAACTTACTTTTTGCCC | Start: 7 |
| Tm | 60.33 | 59.819 | End: 173 |
| Size | 20 | 25 | 167 |
| Primer 3 details |
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| Sequence | GGGGAGGCTAGGGAGTTAGA | ACCTCTCTAACTTACTTTTTGCCCT | Start: 11 |
| Tm | 59.438 | 59.927 | End: 172 |
| Size | 20 | 25 | 162 |
| Primer 4 details |
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| Sequence | GAGGGGAGGCTAGGGAGTTA | ACCTCTCTAACTTACTTTTTGCCCT | Start: 9 |
| Tm | 59.438 | 59.927 | End: 172 |
| Size | 20 | 25 | 164 |
| Primer 5 details |
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| Sequence | AGGGGAGGCTAGGGAGTTAG | ACCTCTCTAACTTACTTTTTGCCCT | Start: 10 |
| Tm | 59.436 | 59.927 | End: 172 |
| Size | 20 | 25 | 163 |
