Primer Details for TRINITY_DN7534_c1_g1_i1 |
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| (AT)8: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGAGTTCAAATCCTTCACTCCA | ACAATCATGGCACGGATAAATAAACT | Start: 55 |
| Tm | 59.652 | 59.679 | End: 225 |
| Size | 24 | 26 | 171 |
| Primer 2 details |
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| Sequence | TCAAATCCTTCACTCCATGTGT | ACAATCATGGCACGGATAAATAAACT | Start: 62 |
| Tm | 57.628 | 59.679 | End: 225 |
| Size | 22 | 26 | 164 |
| Primer 3 details |
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| Sequence | GTTCAAATCCTTCACTCCATGTGT | ACAATCATGGCACGGATAAATAAACT | Start: 60 |
| Tm | 59.48 | 59.679 | End: 225 |
| Size | 24 | 26 | 166 |
| Primer 4 details |
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| Sequence | TCTGAGTTCAAATCCTTCACTCCA | ACAATCATGGCACGGATAAATAAAC | Start: 55 |
| Tm | 59.652 | 58.496 | End: 225 |
| Size | 24 | 25 | 171 |
| Primer 5 details |
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| Sequence | TCAAATCCTTCACTCCATGTGT | ACAATCATGGCACGGATAAATAAAC | Start: 62 |
| Tm | 57.628 | 58.496 | End: 225 |
| Size | 22 | 25 | 164 |
