ssr_primer
Primer Details for TRINITY_DN7230_c0_g1_i3 |
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| (TTC)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGTGTGGCTACATTGCTCT | CAACAGCAAGCTCACAACCC | Start: 461 |
| Tm | 60.035 | 59.969 | End: 674 |
| Size | 20 | 20 | 214 |
| Primer 2 details |
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| Sequence | AGGGTGTGGCTACATTGCTC | CAACAGCAAGCTCACAACCC | Start: 460 |
| Tm | 60.035 | 59.969 | End: 674 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
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| Sequence | GCTTGAGGTGCTTGACTTGC | AGAACTTCCCATGCCCCATG | Start: 1514 |
| Tm | 60.04 | 60.033 | End: 1650 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
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| Sequence | GCTTGAGGTGCTTGACTTGC | GTCGAGGCAAGAACTTCCCA | Start: 1514 |
| Tm | 60.04 | 59.965 | End: 1659 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
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| Sequence | GGGTTGTGAGCTTGCTGTTG | AAGGACACCGCCAAAATCCT | Start: 655 |
| Tm | 59.969 | 59.888 | End: 904 |
| Size | 20 | 20 | 250 |
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