ssr_primer
Primer Details for TRINITY_DN7192_c0_g1_i2 |
|---|
| (T)12gcctaaagtatcccattagttaacctccatt(TC)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCATCTCATCTGACTCCC | AAACTGGTCGAATGGGTCCC | Start: 675 |
| Tm | 59.967 | 59.962 | End: 901 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
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| Sequence | GAGATTGAGGCCGACCGATT | GGGAGTCAGATGAGATGCCG | Start: 595 |
| Tm | 59.895 | 59.967 | End: 694 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
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| Sequence | TGCGTTGAAGGTTGTGGAGT | AAACTGGTCGAATGGGTCCC | Start: 640 |
| Tm | 60.107 | 59.962 | End: 901 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
|---|
| Sequence | AGTGCGTTGAAGGTTGTGGA | AAACTGGTCGAATGGGTCCC | Start: 638 |
| Tm | 60.107 | 59.962 | End: 901 |
| Size | 20 | 20 | 264 |
| Primer 5 details |
|---|
| Sequence | GTCGACAGGAACACTCTCGG | AAACTGGTCGAATGGGTCCC | Start: 658 |
| Tm | 60.11 | 59.962 | End: 901 |
| Size | 20 | 20 | 244 |
(TA)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCATCTCATCTGACTCCC | AAACTGGTCGAATGGGTCCC | Start: 675 |
| Tm | 59.967 | 59.962 | End: 901 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
|---|
| Sequence | GAGATTGAGGCCGACCGATT | GGGAGTCAGATGAGATGCCG | Start: 595 |
| Tm | 59.895 | 59.967 | End: 694 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | TGCGTTGAAGGTTGTGGAGT | AAACTGGTCGAATGGGTCCC | Start: 640 |
| Tm | 60.107 | 59.962 | End: 901 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
|---|
| Sequence | AGTGCGTTGAAGGTTGTGGA | AAACTGGTCGAATGGGTCCC | Start: 638 |
| Tm | 60.107 | 59.962 | End: 901 |
| Size | 20 | 20 | 264 |
| Primer 5 details |
|---|
| Sequence | GTCGACAGGAACACTCTCGG | AAACTGGTCGAATGGGTCCC | Start: 658 |
| Tm | 60.11 | 59.962 | End: 901 |
| Size | 20 | 20 | 244 |
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