ssr_primer
Primer Details for TRINITY_DN7112_c0_g1_i4 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTAGGTTTCGTGGTCGGTGT | CGGAGGGCCAGAAAACTCTT | Start: 157 |
| Tm | 59.967 | 59.963 | End: 376 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
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| Sequence | CCGTCAAGTCGAGATGGGAG | CGGAGGGCCAGAAAACTCTT | Start: 138 |
| Tm | 59.899 | 59.963 | End: 376 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | GTAGGTTTCGTGGTCGGTGT | ACCAACAACGGGAAGCAAGA | Start: 157 |
| Tm | 59.967 | 60.107 | End: 291 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | TCTTGCTTCCCGTTGTTGGT | CGGAGGGCCAGAAAACTCTT | Start: 272 |
| Tm | 60.107 | 59.963 | End: 376 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
|---|
| Sequence | AGGTTTCGTGGTCGGTGTTT | CGGAGGGCCAGAAAACTCTT | Start: 159 |
| Tm | 60.108 | 59.963 | End: 376 |
| Size | 20 | 20 | 218 |
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