Primer Details for TRINITY_DN6856_c0_g1_i1 |
|---|
| (A)10gagagagaaaaatactcaaacaatgtctaccataatctaaaccaaactattaaattgaactaactaaataaaaataacttcatataataagg(A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACACAACCACTAAGGCCAT | TGGTAGACATTGTTTGAGTATTTTTCT | Start: 365 |
| Tm | 57.324 | 57.65 | End: 485 |
| Size | 20 | 27 | 121 |
| Primer 2 details |
|---|
| Sequence | AACACAACCACTAAGGCCAT | TGGTAGACATTGTTTGAGTATTTTTCT | Start: 365 |
| Tm | 57.324 | 57.65 | End: 587 |
| Size | 20 | 27 | 223 |
| Primer 3 details |
|---|
| Sequence | CCATAACACAACCACTAAGGCC | TGGTAGACATTGTTTGAGTATTTTTCT | Start: 361 |
| Tm | 59.247 | 57.65 | End: 485 |
| Size | 22 | 27 | 125 |
| Primer 4 details |
|---|
| Sequence | CCATAACACAACCACTAAGGCC | TGGTAGACATTGTTTGAGTATTTTTCT | Start: 361 |
| Tm | 59.247 | 57.65 | End: 587 |
| Size | 22 | 27 | 227 |
| Primer 5 details |
|---|
| Sequence | AACACAACCACTAAGGCCAT | ACATTGTTTGAGTATTTTTCTCTCTCT | Start: 365 |
| Tm | 57.324 | 57.434 | End: 479 |
| Size | 20 | 27 | 115 |
