Primer Details for TRINITY_DN6854_c1_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCAAATCTTATTGATTAGTCGGCA | TGGTACAGTGTCATCATAGGAAAGA | Start: 40 |
| Tm | 59.469 | 59.28 | End: 141 |
| Size | 25 | 25 | 102 |
| Primer 2 details |
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| Sequence | ACCCAAATCTTATTGATTAGTCGGC | TGGTACAGTGTCATCATAGGAAAGA | Start: 39 |
| Tm | 59.469 | 59.28 | End: 141 |
| Size | 25 | 25 | 103 |
| Primer 3 details |
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| Sequence | TGTTCCTTCTCTCTTAACCCAAA | TGGTACAGTGTCATCATAGGAAAGA | Start: 23 |
| Tm | 57.36 | 59.28 | End: 141 |
| Size | 23 | 25 | 119 |
| Primer 4 details |
|---|
| Sequence | CCCAAATCTTATTGATTAGTCGGCA | TGGTACAGTGTCATCATAGGAAAG | Start: 40 |
| Tm | 59.469 | 58.022 | End: 141 |
| Size | 25 | 24 | 102 |
| Primer 5 details |
|---|
| Sequence | ACCCAAATCTTATTGATTAGTCGGC | TGGTACAGTGTCATCATAGGAAAG | Start: 39 |
| Tm | 59.469 | 58.022 | End: 141 |
| Size | 25 | 24 | 103 |
