Primer Details for TRINITY_DN670_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTAGTTTTGACATGAGTGATGA | AGGGGGAAAAGGAAGGTCTT | Start: 152 |
| Tm | 58.462 | 58.152 | End: 266 |
| Size | 24 | 20 | 115 |
| Primer 2 details |
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| Sequence | ACGTAGTTTTGACATGAGTGATGA | AAGGGGGAAAAGGAAGGTCT | Start: 152 |
| Tm | 58.462 | 58.152 | End: 267 |
| Size | 24 | 20 | 116 |
| Primer 3 details |
|---|
| Sequence | CGTAGTTTTGACATGAGTGATGAGT | AGGGGGAAAAGGAAGGTCTT | Start: 153 |
| Tm | 59.365 | 58.152 | End: 266 |
| Size | 25 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | CGTAGTTTTGACATGAGTGATGAGT | AAGGGGGAAAAGGAAGGTCT | Start: 153 |
| Tm | 59.365 | 58.152 | End: 267 |
| Size | 25 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | ACGTAGTTTTGACATGAGTGATGAG | AGGGGGAAAAGGAAGGTCTT | Start: 152 |
| Tm | 59.365 | 58.152 | End: 266 |
| Size | 25 | 20 | 115 |
