ssr_primer
Primer Details for TRINITY_DN6449_c0_g1_i1 |
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| (AAT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCACTTCGAACATGCCAAC | CGTGGCTCTTCCGATCTTGT | Start: 489 |
| Tm | 59.048 | 60.109 | End: 678 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
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| Sequence | ACCACTTCGAACATGCCAAC | ACGTGGCTCTTCCGATCTTG | Start: 489 |
| Tm | 59.048 | 60.109 | End: 679 |
| Size | 20 | 20 | 191 |
| Primer 3 details |
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| Sequence | GGGGGAAAACCACTTCGAAC | CGTGGCTCTTCCGATCTTGT | Start: 481 |
| Tm | 59.043 | 60.109 | End: 678 |
| Size | 20 | 20 | 198 |
| Primer 4 details |
|---|
| Sequence | GGGGGAAAACCACTTCGAAC | ACGTGGCTCTTCCGATCTTG | Start: 481 |
| Tm | 59.043 | 60.109 | End: 679 |
| Size | 20 | 20 | 199 |
| Primer 5 details |
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| Sequence | ACCACTTCGAACATGCCAAC | GACGTGGCTCTTCCGATCTT | Start: 489 |
| Tm | 59.048 | 59.826 | End: 680 |
| Size | 20 | 20 | 192 |
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