ssr_primer
Primer Details for TRINITY_DN6427_c0_g1_i1 |
|---|
| (GAA)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAACGTCTTGCTCTTGCCC | GCTCCACTGCCGTTTTGAAG | Start: 541 |
| Tm | 60.04 | 60.04 | End: 710 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | GCGATCGTCTCAATGTTCGC | GGGCAAGAGCAAGACGTTTG | Start: 429 |
| Tm | 60.044 | 60.04 | End: 560 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | GCGCGATCGTCTCAATGTTC | GGGCAAGAGCAAGACGTTTG | Start: 427 |
| Tm | 60.044 | 60.04 | End: 560 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | CTTCAAAACGGCAGTGGAGC | GGTGTTGGACTGCACACAAC | Start: 691 |
| Tm | 60.04 | 59.899 | End: 927 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
|---|
| Sequence | GGCATTGCCTTCCTCTCCTT | TTTCCGCTCCATCCATGACC | Start: 115 |
| Tm | 60.034 | 60.107 | End: 353 |
| Size | 20 | 20 | 239 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAACGTCTTGCTCTTGCCC | GCTCCACTGCCGTTTTGAAG | Start: 541 |
| Tm | 60.04 | 60.04 | End: 710 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | GCGATCGTCTCAATGTTCGC | GGGCAAGAGCAAGACGTTTG | Start: 429 |
| Tm | 60.044 | 60.04 | End: 560 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | GCGCGATCGTCTCAATGTTC | GGGCAAGAGCAAGACGTTTG | Start: 427 |
| Tm | 60.044 | 60.04 | End: 560 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | CTTCAAAACGGCAGTGGAGC | GGTGTTGGACTGCACACAAC | Start: 691 |
| Tm | 60.04 | 59.899 | End: 927 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
|---|
| Sequence | GGCATTGCCTTCCTCTCCTT | TTTCCGCTCCATCCATGACC | Start: 115 |
| Tm | 60.034 | 60.107 | End: 353 |
| Size | 20 | 20 | 239 |
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