ssr_primer
Primer Details for TRINITY_DN6221_c0_g1_i2 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGTGGAGGCTTCGAAATT | AATTCATGCGCCGATGAAGC | Start: 776 |
| Tm | 59.964 | 59.97 | End: 1006 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
|---|
| Sequence | AACGGTGGAGGCTTCGAAAT | AATTCATGCGCCGATGAAGC | Start: 775 |
| Tm | 59.964 | 59.97 | End: 1006 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
|---|
| Sequence | GCTTCATCGGCGCATGAATT | GTAGAACCTGTTGAGCGGCT | Start: 987 |
| Tm | 59.97 | 60.037 | End: 1152 |
| Size | 20 | 20 | 166 |
| Primer 4 details |
|---|
| Sequence | GCTTCATCGGCGCATGAATT | AGTAGAACCTGTTGAGCGGC | Start: 987 |
| Tm | 59.97 | 60.037 | End: 1153 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | AGCGTGAACGTGACCTTGAT | ATTTCGAAGCCTCCACCGTT | Start: 607 |
| Tm | 59.966 | 59.964 | End: 794 |
| Size | 20 | 20 | 188 |
(AT)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGTGGAGGCTTCGAAATT | AATTCATGCGCCGATGAAGC | Start: 776 |
| Tm | 59.964 | 59.97 | End: 1006 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
|---|
| Sequence | AACGGTGGAGGCTTCGAAAT | AATTCATGCGCCGATGAAGC | Start: 775 |
| Tm | 59.964 | 59.97 | End: 1006 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
|---|
| Sequence | GCTTCATCGGCGCATGAATT | GTAGAACCTGTTGAGCGGCT | Start: 987 |
| Tm | 59.97 | 60.037 | End: 1152 |
| Size | 20 | 20 | 166 |
| Primer 4 details |
|---|
| Sequence | GCTTCATCGGCGCATGAATT | AGTAGAACCTGTTGAGCGGC | Start: 987 |
| Tm | 59.97 | 60.037 | End: 1153 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | AGCGTGAACGTGACCTTGAT | ATTTCGAAGCCTCCACCGTT | Start: 607 |
| Tm | 59.966 | 59.964 | End: 794 |
| Size | 20 | 20 | 188 |
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