Primer Details for TRINITY_DN616_c0_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGGAATTAATCATCCAAAACTGA | AGAGATATCTGTGCAGGCCA | Start: 205 |
| Tm | 58.74 | 58.201 | End: 327 |
| Size | 25 | 20 | 123 |
| Primer 2 details |
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| Sequence | TGGGGAATTAATCATCCAAAACTGA | AGATATCTGTGCAGGCCAACT | Start: 205 |
| Tm | 58.74 | 59.157 | End: 325 |
| Size | 25 | 21 | 121 |
| Primer 3 details |
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| Sequence | TGGGGAATTAATCATCCAAAACTGA | GCAGGCCAACTAAAACTCACA | Start: 205 |
| Tm | 58.74 | 59.046 | End: 315 |
| Size | 25 | 21 | 111 |
| Primer 4 details |
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| Sequence | TGGGGAATTAATCATCCAAAACTGA | TGCAGGCCAACTAAAACTCAC | Start: 205 |
| Tm | 58.74 | 59.046 | End: 316 |
| Size | 25 | 21 | 112 |
| Primer 5 details |
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| Sequence | TGGGGAATTAATCATCCAAAACTGA | GTGCAGGCCAACTAAAACTCA | Start: 205 |
| Tm | 58.74 | 59.046 | End: 317 |
| Size | 25 | 21 | 113 |
