ssr_primer
Primer Details for TRINITY_DN5910_c0_g1_i1 |
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| (GCC)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGAAGAGGGAGACGCCAT | TTTTCGTCGGCTTGCTTTGG | Start: 2180 |
| Tm | 60.033 | 59.97 | End: 2310 |
| Size | 20 | 20 | 131 |
| Primer 2 details |
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| Sequence | TTGTAGCCTTGTTGCAACGC | ATGGCGTCTCCCTCTTCTCT | Start: 1957 |
| Tm | 59.97 | 60.033 | End: 2199 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
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| Sequence | TTGTAGCCTTGTTGCAACGC | ACTTCGAAGTCATGGCTGCA | Start: 1957 |
| Tm | 59.97 | 59.965 | End: 2236 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
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| Sequence | CCAAAGCAAGCCGACGAAAA | GGGGCTGTTCTCAAGCTCTT | Start: 2291 |
| Tm | 59.97 | 59.963 | End: 2494 |
| Size | 20 | 20 | 204 |
| Primer 5 details |
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| Sequence | AGGTTGAGAAGCGTGAGTGG | GGGGCTGTTCTCAAGCTCTT | Start: 2250 |
| Tm | 59.966 | 59.963 | End: 2494 |
| Size | 20 | 20 | 245 |
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