ssr_primer
Primer Details for TRINITY_DN5823_c1_g1_i1 |
|---|
| (A)18: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCATGCAAGCAGCCTTTG | CAGCGCGGATCATTGACAAG | Start: 231 |
| Tm | 59.966 | 59.973 | End: 444 |
| Size | 20 | 20 | 214 |
| Primer 2 details |
|---|
| Sequence | GAATGGCTCCGAGTTCCCAA | CAGCGCGGATCATTGACAAG | Start: 296 |
| Tm | 60.035 | 59.973 | End: 444 |
| Size | 20 | 20 | 149 |
| Primer 3 details |
|---|
| Sequence | TCAGAGGCTGCGTTGGAATT | CAGCGCGGATCATTGACAAG | Start: 345 |
| Tm | 59.963 | 59.973 | End: 444 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
|---|
| Sequence | GGGAAACGGGTTTTTGCTCC | CAAAGGCTGCTTGCATGGTT | Start: 122 |
| Tm | 59.967 | 59.966 | End: 250 |
| Size | 20 | 20 | 129 |
| Primer 5 details |
|---|
| Sequence | GGGAAACGGGTTTTTGCTCC | TTGGGAACTCGGAGCCATTC | Start: 122 |
| Tm | 59.967 | 60.035 | End: 315 |
| Size | 20 | 20 | 194 |
(AGC)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCATGCAAGCAGCCTTTG | CAGCGCGGATCATTGACAAG | Start: 231 |
| Tm | 59.966 | 59.973 | End: 444 |
| Size | 20 | 20 | 214 |
| Primer 2 details |
|---|
| Sequence | GAATGGCTCCGAGTTCCCAA | CAGCGCGGATCATTGACAAG | Start: 296 |
| Tm | 60.035 | 59.973 | End: 444 |
| Size | 20 | 20 | 149 |
| Primer 3 details |
|---|
| Sequence | TCAGAGGCTGCGTTGGAATT | CAGCGCGGATCATTGACAAG | Start: 345 |
| Tm | 59.963 | 59.973 | End: 444 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
|---|
| Sequence | GGGAAACGGGTTTTTGCTCC | CAAAGGCTGCTTGCATGGTT | Start: 122 |
| Tm | 59.967 | 59.966 | End: 250 |
| Size | 20 | 20 | 129 |
| Primer 5 details |
|---|
| Sequence | GGGAAACGGGTTTTTGCTCC | TTGGGAACTCGGAGCCATTC | Start: 122 |
| Tm | 59.967 | 60.035 | End: 315 |
| Size | 20 | 20 | 194 |
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