Primer Details for TRINITY_DN5787_c0_g1_i1 |
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| (TTA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTATGGTCTGATATCATCTTCCA | TGGAAACTAAAAATAGCGTTTTGACC | Start: 45 |
| Tm | 59.729 | 59.081 | End: 259 |
| Size | 26 | 26 | 215 |
| Primer 2 details |
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| Sequence | TGACATGCTTATGGTCTGATATCA | TGGAAACTAAAAATAGCGTTTTGACC | Start: 40 |
| Tm | 57.576 | 59.081 | End: 259 |
| Size | 24 | 26 | 220 |
| Primer 3 details |
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| Sequence | TCTATTGACATGCTTATGGTCTGA | TGGAAACTAAAAATAGCGTTTTGACC | Start: 35 |
| Tm | 57.516 | 59.081 | End: 259 |
| Size | 24 | 26 | 225 |
| Primer 4 details |
|---|
| Sequence | TGCTTATGGTCTGATATCATCTTCCA | GGAAACTAAAAATAGCGTTTTGACCT | Start: 45 |
| Tm | 59.729 | 58.857 | End: 258 |
| Size | 26 | 26 | 214 |
| Primer 5 details |
|---|
| Sequence | TGCTTATGGTCTGATATCATCTTCCA | TGGAAACTAAAAATAGCGTTTTGACCT | Start: 45 |
| Tm | 59.729 | 60.202 | End: 259 |
| Size | 26 | 27 | 215 |
